The Anti-tumor Activity of Vitamin C via the Increase of Fas (CD95) and MHC I Expression on Human Stomach Cancer Cell Line, SNU1.
- Author:
Yeonsil YU
1
;
Seyeon BAE
;
Hyemin KIM
;
Yejin KIM
;
Nag Bum CHU
;
Nag Kyun CHU
;
Jae Seung KANG
;
Wang Jae LEE
Author Information
- Publication Type:Original Article
- Keywords: Vitamin C; Antitumor activity; Stomach cancer; Fas
- MeSH: Apoptosis; Ascorbic Acid; Cell Line; Humans; Stomach; Stomach Neoplasms; Vitamins
- From:Immune Network 2011;11(4):210-215
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: It is already known that high concentration of vitamin C induces apoptosis on tumor cells. However, there is no report regarding the function of vitamin C on the modulation of immune susceptibility of cancer. Therefore, we investigated whether vitamin C can modulate immune susceptibility of tumor cells, especially on the induction of Fas-mediated apoptosis. METHODS: First, the optimal concentration of vitamin C, which cannot induce damages on tumor cells for 36 hrs. We found that 2 mM of vitamin C did not show harmful effect. In addition, the optimal concentration of agonistic anti-Fas Abs for 18 hrs was examined. RESULTS: As a result, 400 ng/ml of agonistic anti-Fas Abs did not induce apoptosis on tumor cells. Next, we tried to find the effect of 2 mM of vitamin C on the modulation of the susceptibility to agonistic anti-Fas Abs. When tumor cells were cultured with 400 ng/ml of agonistic anti-Fas Abs for 18 hrs, after pre-treatment with 2 mM of vitamin C for 24 hrs, viability of cells was decreased. Interestingly, we found that the expression of Fas (CD95) and MHC class I was increased by the treatment of vitamin C. CONCLUSION: Taken together, vitamin C increases the susceptibility of tumor cells to anti-Fas Abs and the expression of Fas (CD95) and MHC class I on tumor cells.
