The role of IL-6 in the co-cultivation of porcine hepatocytes with bone marrow mesenchymal stem cells

VernacularTitle:IL-6在猪肝细胞与骨髓间充质干细胞共培养中的作用
Author: Jinyang GU ; Xiaolei SHI ; Yue ZHANG ; Yitao DING
Publication Type:Journal Article
Keywords: Interleukin-6; Mesenchymal stem cells; Hepatocytes; Swine
From: Chinese Journal of General Surgery 2010;25(1):53-55
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of IL-6 in the co-cultivation system of porcine hepatocytes with bone marrow mesenchymal stem cells (MSCs) in vitro.Methods Mononuclear cells were isolated from bone marrow aspirate of swines (n = 3) by 1.077 g/ml density gradient centrifugation.MSCs of passage 3 and primary hepatocytes harvested by a two-step in situ collagenase perfusion technique were inoculated in Millicell culture inserts at a seeding ratio of 1:2,and functional changes of heterotypic interactions were characterized.Levels of IL-6,TNF-α,and TGF-α were measured respectively.Results Hepatocyte viability was greater than 95%.Hepatocyte performance levels such as albumin secretion and urea synthesis were all significantly enhanced in co-culture group compared with hepatocyte homo-culture (P＜0.05).The IL-6 level also significantly increased in co-culture group (P ＜ 0.01).A significant decrease of hepatic functions was observed upon neutralization of IL-6 in co-culture.Conclusion MSC-derived IL-6 plays a key role in the up-regulation of hepatocyte functions in this co-culture.