Expression of decorin and transforming growth factor-β_1 mRNA and type Ⅰ collagen in the anterior vagina of women with pelvic organ prolapse
10.3760/cma.j.issn.1673-4904.2010.03.001
- VernacularTitle:盆腔器官脱垂患者阴道壁核心蛋白聚糖和转化生长因子-β_1mRNA表达水平及胶原含量的研究
- Author:
Yan YU
;
Yanfeng SONG
- Publication Type:Journal Article
- Keywords:
Urinary incontinence;
Collagen type Ⅰ;
Proteoglycans;
Transforming growth factors;
Pelvic organ prolapse
- From:
Chinese Journal of Postgraduates of Medicine
2010;33(3):1-3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine quantity of type Ⅰ collagen and mRNA expression of decorin and transforming growth factor (TGF)- β_1 in the upper portion of the anterior vagina in women with pelvic organ prolapse (POP). Methods Transvaginat biopsies were obtained from the anterior vaginal wall in 27 POP(POP group) and 21 non-poP(control group). The concentration of type Ⅰ collagen was determined by ELISA technique. RT-qPCR was used to verify the mRNA level of decorin and TGF- β_1. Results The mean concentration of type Ⅰ collagen in the premenopausal women in POP group [(47.94 ± 6.82) mg/g total protein (TP)]was significantly lower than that in control group [(62.33±6.57) mg/g TP],in the postmenopausal women in POP group, it was (41.82±5.96) mg/g TP, significantly lower than that in control group[(61.21 ± 4.84)mg/g TP](P< 0.01). The mRNA expression of decorin was 1.61 ± 0.10 in POP group, significantly lower than that in control group 2.77±0.60 ,while that of TGF-β_1 was higher in POP greup10.86 ± 0.23 than that in control group 9.87 ± 0.92 (P < 0.05). In the postmenopausal women in two groups, there were no significant difference in the mRNA expression of decorin and TGF-β_1 (P > 0.05). Conclusions A decrease in quantity of type Ⅰ coUagen in the connective tissue of anterior vagina may compromise the tensile strength and an increase susceptibihty to prolapse. Deeorin might be involved in the pathogenesis in premenopausal women with POP. The pathogenesis of POP is muhifactorial.
