Th1/Th2 cytokine balance in patients with severe chronic hepatitis B and its relationship with prognosis
10.3760/cma.j.issn.1674-2397.2009.05.003
- VernacularTitle:慢性重型乙型肝炎Th1/Th2平衡状态与预后关系的研究
- Author:
Bingliang LIN
;
Yubo HUANG
;
Xiaohong ZHANG
;
Junqiang XIE
;
Shaoquan ZHANG
;
Huijuan CAO
;
Yutian CHONG
;
Zhiliang GAO
- Publication Type:Journal Article
- Keywords:
Chronic hepatitis B;
Cytokine;
Prognosis
- From:
Chinese Journal of Clinical Infectious Diseases
2009;2(5):264-267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the balance of Th1/Th2 cytokines and its relationship with prognosis of severe chronic hepatitis B ( CHB ). Methods Peripheral blood samples were collected from 112 severe CHB patients, 30 CHB patients and 30 healthy controls. IL-4, IFN-γ levels and HBV DNA loads were measured by ELISA and fluorescent PCR, respectively. The levels of cytokines in different stages, and their correlations with HBV DNA loads and short-term prognosis were analyzed. Results Higher levels of IL-4, IFN-γ and Th1/Th2 ratios in peripheral blood were detected in patients with severe CHB than those with CHB and the healthy controls (Z = 8.968, 10. 004 and 26. 067, P =0. 009, 0. 007 and 0. 000). IL4 levels in patients with end-stage server CHB were markedly higher than those in other stages ( Z = 3. 672 and 3. 158, P= 0.000 and 0.002), while their Thl/Th2 ratios were lower (Z=3. 161 and 2. 166, P=0.002 and 0. 030). No significant differences on levels of IL-4, IFN-γ and Th1/Th2 ratios were observed in severe CHB patients with different HBV DNA levels (Z =4.431, 2.626 and 0. 140, P =0.219, 0.403 and 0. 987). Elevated IL-4 was closely correlated with the high case-fatality rate within 12 weeks. Conclusions The balance between Th1 and Th2 cytokines is- disturbed in patients with severe CHB. Thl/Th2 ratio decreases with the aggravation of diseases, which may indicate unfavorable short-term prognosis.
