Effect of on adhesion between pancreatic stellate cell and mast cell
10.3760/cma.j.issn.1674-1935.2009.05.010
- VernacularTitle:干细胞因子在胰腺星状细胞和肥大细胞黏附中的作用
- Author:
Kai WU
;
Gang XU
;
Mingyi XU
;
Xingpeng WANG
- Publication Type:Journal Article
- Keywords:
Stem cell factor;
Pancreas;
Astracytesl;
Mast cells
- From:
Chinese Journal of Pancreatology
2009;9(5):318-320
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate stem cell factor(SCF)expression in activated pancreatic stellate cell(PSC)and the effect of SCF on adhesion between PSC and mast cell(MC).Methods PSC of SD rat was separated and activated by pancreatic tissue culture;the expression of SCF mRNA and protein was detected bv RT-PCR and immunachemistry.SCF was blocked with different dose of anti-SCF antibody,the rate of adhesion between PSC and MC was determined by thiomn blue stain and β-hexasaminidase assay.PSC was stimulated by 10 μg/ml mitomycin-C and was co-cultured with MC,then the growth of MC was observed at 2,3,5 day,respectively.Results Activated PSC expressed SCF mRNA and protein,the number of adhered MC in the control,1mg/ml and 10 mg/ml antibody groups was 48±10,28±8,and 23±9,respectively;the difference between antibody and control group was statistically significant(P＜0.05).One day after PSC and MC was co-cultured without serum.there were MC adhesion;3 days later the rate of adhesion increased;5 days later the adhesion appeared bulk-like and lots of colonies were present,while PSC basically disappeared.Conclusions Activated PSC may synthesize,express SCF and induced adhesion and growth of MC.
