XAF1 gene mediated by recombinant adenovirus induces apoptosis in pancreatic cell line BxPC-3
10.3760/cma.j.issn.1674-1935.2009.05.016
- VernacularTitle:XAF1基因诱导胰腺癌细胞株BxPC3凋亡的实验研究
- Author:
Jia HUANG
;
Qi ZHU
;
Haixia CAO
;
Weiyan YAO
;
Yongping ZHANG
;
Yaozong YUAN
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Adenoviruses;
XAF1 genes;
Apoptosis
- From:
Chinese Journal of Pancreatology
2009;9(5):337-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether expression of XAF1 mediated by edenovirus vector AdS/F35 could induce apoptosis in pancreatic cancer cell line BxPC-3 and its possible mechanisms.Methods Preconstructed recombinant Ad5/F35-XAF1 virus and negative control Ad5/F35-Null was tranfected into BxPC3:the expression of XAF1 mRNA and protein before and after tranfection was,analyzed by semi-quantitative RT-PCR and Western blot.The expressions of proteins including Caspase-3,PARP,Caspase-8 and bcl-2 were detected by Western blots.Cell apoptosis was assessed by Annexin-v/PI and TUNEL staining.Results After Ad5/F35-XAF1 tranfection,XAF1 mRNA and protein expression significantly increased,and the difference was statistically significant when compared with control group and Ad5/F35-Null group(P＜0.05).The apoptosis rate was(19.90±3.09)%and(9.29±2.13)%,which was significantly different(P＜0.01)from those in the Ad5/F35-Null group[(6.72±0.7)6%,(2.73±0.51)%]or in the control group[(7.22±1.53)%,(1.56±0.47)%].The expression of Caspase-3,PARP and Caspase-8 significantly increased,but the expression of bcl-2 decreased.Conclusions XAF1 plays a major role in the apoptosis of pancreatic cancer that acts thriugh the activation of death receptor pathway and mitochondrial pathway of apoptosis.
