Analysis of differential expression of serum proteins in concanavalin A induced liver injury mouse model
10.3760/cma.j.issn.1000-6680.2009.12.001
- VernacularTitle:刀豆蛋白A诱导小鼠肝损伤模型的血清蛋白质差异分析
- Author:
Xufei TAN
;
Feng CHEN
;
Shanshan WU
;
Guoping PENG
;
Jing WANG
;
Zhi CHEN
- Publication Type:Journal Article
- Keywords:
Concanavalin A;
Liver diseases;
Disease models;
animal;
Electrophoresis;
gel;
two dimensional;
Protein precursor
- From:
Chinese Journal of Infectious Diseases
2009;27(12):705-709
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the differential expression of serum proteins in concanavalin A(ConA) induced liver injury mouse model and analyze the relationship between disease progression and special proteins. Methods Twenty-five male mice were randomly divided into five groups, i.e. blank control group, phosphate-buffered saline (PBS) control group, liver injury groups developed 1, 3 and 6 h after ConA injection. The sera from all five groups were removed off the albumin by ProteoExtract~(TM)albumin removal kit. Then two dimensional electrophoresis ( 2DE) and mass spectrometry analysis were utilized to identify differences in protein expressions. Results Two specific proteins were detected in the liver injury group developed 6 h after ConA injection, which were identified as serum amyloid A-2 protein precursor and serum amyloid A-l protein precursor by mass spectrometry. Conclusions Serum amyloid A-2 protein precursor and serum amyloid A-l protein precursor are found at 6 h of ConA injection in ConA induced liver injury mouse model, which may be related to disease progression.
