Immunoprotection analysis of hepatitis C virus recombinant protein
10.3760/cma.j.issn.1000-6680.2010.01.006
- VernacularTitle:丙型肝炎病毒重组蛋白的免疫保护性
- Author:
Guangxue LI
;
Ruihong ZENG
;
Shigan LING
;
Heqiu ZHANG
;
Zhiyan YAO
;
Lin WEI
- Publication Type:Journal Article
- Keywords:
Hepacivirus;
Hepatitis C;
Viral hepatitis vaccines;
Antibody formation;
Immunity;
cellular;
Recombinant proteins
- From:
Chinese Journal of Infectious Diseases
2010;28(1):19-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate immune responses and protective effect induced by two recombinant proteins of hepatitis C virus(HCV)in BALB/c mice.Methods BALB/c mice were immunized with recombinant proteins HCV-T and(or)HCV-F4HVR1 three times.Specific antibodies in sera were tested by enzyme-linked immunosorbent assay(ELISA).Five mice were sacrificed after 14 days of the last immunization.Splenic cells were isolated and levels of interferon(IFN)-γ,interleukin(IL)-4 and cytotoxic T lymphocyte(CTL)cytotoxicity assay were measured in vitro.The remaining mice were subcutaneously injected with 1.0×106 SP2/0-NS3 cells on the back to investigate the protective effects.The differences of means between groups were compared by LSD-t test.Results Compared with phosphate bufter saline(PBS)group,combined immunization with HCV-T and HCV-F4HVR1 induced higher levels of specific IgG against HCV-F4HVR1(t=3.815,3.762,P＜0.05),HCV-NS3-specific CTL response(t=3.971,P＜0.05)and IL-4(t=3.512,3.417,P＜0.05)and IFN-γ(t=3.813,3.426,3.671,P＜0.05)secretions.Conclusion High levels of specific humoral immunity and cellular immunity are induced in vivo after combined immunization with HCV-T and HCV-F4 HVR1,which could effectively prevent from the attack of SP2/0-NS3 cells.
