Regulation of pancreatic cancer cells by fibroblast growth factor receptor-1

VernacularTitle:成纤维细胞生长因子受体1在胰腺癌细胞中的表达和调控
Author: Guowei CHEN ; Zhanbing LIU ; Hongqiao GAO ; Yinmo YANG ; Yuanlian WAN ; Yucun LIU
Publication Type:Journal Article
Keywords: Pancreatic neoplasms; Receptor,fibroblast growth factors,type 1; Protein kinases inhibitors; Signal transduction
From: Chinese Journal of General Surgery 2010;25(1):56-60
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression and regulation of FGFR1 protein and mRNA in human pancreatic cancer cell lines.Methods The expressions of FGFR1 protein and mRNA in pancreatic cancer cells were tested by Western blot,Northern blot and RT-PCR.The effects of exogenous growth factors and tyrosine kinase inhibitors on expression of FGFR1 protein and mRNA was observed.Results FGFR1 protein and mRNA expressed in 7 pancreatic cancer cell lines in different levels.After stimulation of several exogenous growth factors,we found that IGF-1,EGF and FGF2 up-regulated the expression of FGFR1 in Mia PaCa-2 significantly;EGF and FGF2 up-regulated the expression of FGFR1 in PANC-1 significantly (P＜0.05).The effect of FGF2 on the expression of FGFR1 was in time-dependent manner.ERK1/2 special inhibitor UO126 and p38 MAPK special inhibitor SB203580 down-regulated the expression of FGFR1.Conclusion Expression of FGFR1 is up-regulated by growth factors and may be modulated through ERK1/2 and p38 MAPK signal transduction pathway.