Diagnostic value of measurement of SARP2 methylation in peripheral blood for detection of pancreatic cancer
10.3760/cma.j.issn.1674-1935.2009.04.004
- VernacularTitle:外周血SARP2基因甲基化检测对胰腺癌的诊断价值
- Author:
Jian SONG
;
Yiqi DU
;
Shunli LV
;
Jia CAO
;
Jia GAO
;
Zhaoshen LI
;
Jing JIN
;
Yanfang GONG
;
Xiaohua MAN
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
DNA methylation;
SARP2 genes
- From:
Chinese Journal of Pancreatology
2009;9(4):229-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic value of measurement of SARP2 methylation in peripheral blood for detection of pancreatic cancer in human. Methods Peripheral vein blood of 12 patients with primary pancreatic cancers, 10 patients with chronic pancreatitis and 6 health volunteers were collected. Serum free DNA were extracted from blood samples, and were modified with bisulfate, and SARP2 gene extron 1 were amplified through BSP and sequencing of the production. Results There were 12 patients (83 %) with pancreatic cancer and 10 patients (40%) with chronic pancreatitis had obvious methylation in SARP2 gene in peripheral blood. The rate of CpG methylation in SARP2 gene extron 1 of pancreatic cancer, chronic pancreatitis and health volunteers was 16. 8% , 10. 4% and 2. 2% respectively. There was statistically significant difference among the three groups (P<0.01 or P< 0.05). Conclusions Aberrant methylation of SARP2 gene could be detected in peripheral blood in patients with pancreatic cancer, the detection of SARP2 gene methylation may have potential clinical implication for diagnosis of pancreatic cancer.
