Effects of partial radiation in vitro on chromosome aberrations in human peripheral blood lymphocytes

VernacularTitle:部分照射离体血对淋巴细胞染色体畸变形成的影响
Author: Jianxiang LIU ; Jianlei RUAN ; Mei TIAN ; Yan PAN ; Xu SU
Publication Type:Journal Article
Keywords: Partial irradiation; Human peripheral blood; Chromosome aberration
From: Chinese Journal of Radiological Medicine and Protection 2010;30(1):26-28
CountryChina
Language:Chinese
Abstract: Objective To analyze the chromosome aberrations induced by partial radiation in human peripheral blood lymphocytes in vitro.Methods Heparinized whole blood samples were exposed to 2 Gy ~(60)Co 7-rays at 37℃ ,and then mixed with non-irradiated blood by different ratio.The slides were prepared after culturing and the unstable aberrations were analyzed.Results The chromosome aberrations had a good relationship with the ratio of irradiated blood.The chromosome aberrations in partial irradiated group were higher than that in the irradiated group.The estimated dose was 1.27 Gy when the ratio was 1 : 1 ,greater than the dose of 1 Gy.The estimated dose was 0.93 Gy when the ratio was 0.5＝1,also greater than 0.5 Gy.But when the ratio was 1:0,the radiation dose was accordant with the estimated dose.Conclusions Chromosome aberrations could be a biomarker for estimating the uneven irradiation.