mRNA and protein expressions of TGF-β1 in spinal cord injuries and their significance after transplantation of activated microglia

VernacularTitle:活化小胶质细胞移植后损伤脊髓内TGF-β1 mRNA、蛋白的表达及意义
Author: Dewei KOU ; Tengbo YU ; Tianmi LI ; Zhenping YU
Publication Type:Journal Article
Keywords: Spinal curd injuries; Transforming growth factor betal; Cell transplantation; Microglia
From: Chinese Journal of Trauma 2010;26(1):70-75
CountryChina
Language:Chinese
Abstract: Objective To investigate mRNA and protein expressions of TGF-β1 and their signifi-cance in injured spinal cord after transplantation of activated microglia. Methods Wistar rat model of spinal cord injury was made by using modified Allen's method. Then, the activated microglia was trans-planted into spinal cord injury models tu detect mRNA and protein expressions of TGF-β1 in the spinal curd. Results The mRNA and protein expressions of TGF-β1 in control group were significantly lower than that in test group (P<0.05). Conclusion Transplantation of activated microglia to the impaired spinal cord in rats can increase the mRNA and protein expressions of TGF-β1. The up-regulated expres-sion of TGF-β1 in spinal cord may play a role in promoting the recovery of spinal cord, when the activated microglia may be the main saurce of TGF-β1.