Effects of combined use of glycogen synthase kinase-3 inhibitor and fructose-1, 6-diphosphate on liver trauma in rats
10.3760/cma.j.issn.1001-8050.2010.01.024
- VernacularTitle:糖原合成酶激酶-3抑制剂与1,6-二磷酸果糖联合应用对大鼠肝脏创伤的影响
- Author:
Ming LU
;
Lijun TANG
;
Xiaolin MA
- Publication Type:Journal Article
- Keywords:
Wounds and injuries,liver;
Liver glycogen;
Fructosediphosphates;
Glycogen synthase kinase-3;
Reperfusion injury
- From:
Chinese Journal of Trauma
2010;26(1):76-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of combined use of glycogen synthase kinase (GSK) -3 inhibitor and fructose-1,6-diphosphate (FDP) on liver trauma in rats. Methods After crea-tion of liver trauma model in 49 Sprague-Dawley rats, 42 rats were randomly divided into control group (NaCl group), FDP group and FGI Group (FDP and GSK-3 inhibitor in combination group). Then, each group was randomly subdivided into pre-ischemia group and 4-hour reperfusion group on account of time point when animals were sacrificed before and after iachemia. The other seven rats set as sham operation group (SH group) were sacrificed at 4-hour reperfusion time point. The AST and ALT levels in hlood and glycogen content, SOD vitality and MDA content in liver tissues were determined. Results At pre-is-chemia time point, liver glycogen content in three groups was in order of control group < FDP group < FGI group (P <0.01). At 4-hour reperfusion time point, blood ALT and AST levels in four groups were in order of control group > FDP group > FGI group > SH group (P < 0.01), while SOD vitality in liver tissues of four groups was in order of control group < FDP group < FGI group < SH group (P < 0.01) and MDA content in four groups was in order of control group > FDP group > FGI group > SH group (P < 0.01). Conclusions Combined use of FDP and GSK-3 inhibitor can enhance the protective effect of FDP on liver rupture, as may relate to the mechanism that GSK-3 inhibitor can effectively enhance glycogen synthesis of FDP as substrate before liver ischemia so that the liver glycogen storage is increased in a short period of time and hence post-traumatic warm ischemia-reperfusion injury is alleviated in the liver of rats.
