Changes of IL-1β and IL-1ra expression in the hippocampus of rats with frequent febrile seizures

VernacularTitle:反复热性惊厥大鼠海马IL-1β和IL-1ra表达的变化
Author: Feixia ZHENG ; Guangqian LI ; Xulai SHI ; Zhongdong LIN
Publication Type:Journal Article
Keywords: Seizures,febrile; IL-1β; IL-1ra; Hippocampus; Rat
From: Chinese Pediatric Emergency Medicine 2010;17(1):37-39
CountryChina
Language:Chinese
Abstract: Objective To evaluate the changes of IL-1β and IL-lra in hippocampus of rats with frequent febrile seizures(FS).Methods 21-day-old male Sprague-Dawley(SD)rats were randomly divided into 3 groups:normal control group(NC group),hyperthermic control group(HC group)and febrile seizures group(FS group).IL-1β and IL-1ra levels in hippocampus of these rats were determined by ELISA methods,IL-1β mRNA and IL-1ra mRNA levels in hippocampus were measured by RT-PCR.Results IL-1β mRNA and protein levels in hippocampus of rats in FS group were significantly higher than those in NC group and HC group(P＜0.01,P＜0.05).IL-1ra mRNA and protein levels in hippocampus of rats in FS group were significantly higher than those in NC group and HC group(P＜0.01,P＜0.05),too.IL-1ra/IL-1β protein ratio showed no significant difference among 3 groups.Conclusion Frequent FS can cause the increase of IL-1β and IL-lra mRNA and protein levels in hippocampus,suggesting that IL-1β and IL-1ra may be involved in the pathogenesis of hippocampal neuron damage in FS rat.