Effects of gabapentin on high voltage active calcium currents in injured dorsal root ganglion neurons in a rat model of neuropathic pain
10.3760/cma.j.issn.0254-1416.2010.01.017
- VernacularTitle:加巴喷丁对神经病理性痛大鼠背根神经节神经元高电压激活钙电流的影响
- Author:
Xiaodong CHEN
;
Minmin ZHU
;
Shanshan AN
;
Qiang WANG
;
Liang LU
;
Qin SUN
;
Hang XIAO
;
Jianguo XU
;
Manlin DUAN
- Publication Type:Journal Article
- Keywords:
Cyclohexanecarboxylic acids;
Calcium channels;
Neuralgia;
Ganglia,spinal;
Neurons
- From:
Chinese Journal of Anesthesiology
2010;30(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of gabapentin on high voltage active calcium currents in the injured dorsal root ganglion (DRG) neurons in a rat model of neuropathic pain.Methods Pathogen-free male SD rats aged 4-6 weeks were used in this study. The animals were anesthetized with intraperitoneal pentobarbital soclium 50 mg/kg. L_5 spinal nerve was ligated between DRG and sciatic nerve and cut distal to the ligature. The animals were decapitated on the 14th postoperative day. L_5 DRG was isolated and the neurons in the ganglion were enzymatically dissociated. The high voltage active calcium current was recorded using whole-cell patch-clamp technique.Results Gabapentin inhibited the peak calcium current in the injured DEG neurons. Peak calcium current was decreased by gabapentin 100 μmol/L and both activation and steady-state inactivation curve shifted to more hyperpolarized potentials. Conclusion Gabapentin can inhibit high voltage active in the injured DRG neurons in a rat model of neuropathic pain. The alteration in the inactivation of the electrophysiological properties may be involved in the mechanism.
