Cerebral protective effect of anti-intercellular adhesion molecnle-1 antibody in rats with global cerebral ischemia/reperfusion injury
10.3760/cma.j.issn.0254-1416.2010.01.024
- VernacularTitle:细胞间黏附分子-1单克隆抗体对全脑缺血再灌注损伤大鼠的脑保护作用
- Author:
Jianping CAO
;
Xueyin SHI
;
Xiaoyong MIAO
;
Jia XU
- Publication Type:Journal Article
- Keywords:
Intercellular adhesion molecule-1;
Antibodies,monoclonal;
Brain ischemia;
Reperfusion injury
- From:
Chinese Journal of Anesthesiology
2010;30(1):83-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cerebral protective effect of anti-intercellular adhesion molecule-1 (anti-ICAM-1) antibody (1A29) in rats with global cerebral ischemia/reperfusion (I/R) injury. Methods Forty healthy SD rats weighing 180-200 g were randomly divided into 4 groups ( n = 10 each) : group Ⅰ received isotype-matched control antibody 1 mg/kg iv (control group); group Ⅰ ,Ⅲ ,Ⅳ received 1A29 1 mg/kg iv before cerebral ischemia (group Ⅱ), at the onset of reperfusion (group Ⅲ) and at 4 h of reperfusion (group Ⅳ) respectively. The global cerebral I/R was produced by 30 min four-vessel occlusion followed by 24 h reperfusion. All animals were killed at 24 h of reperfusion. Their brains were removed for microscopic examination and measurement of W/D weight ratio, infarct size and neurological deficits (0 = no deficit,4 = unable to crawl with mental depression) .Results The PMN leukocyte and monocyte infiltration, water content of the brain and infarct size were significantly decreased in group Ⅱ ,Ⅲ and Ⅳ as compared with control group. The neurological deficit scores were significantly lower in group Ⅱ ,Ⅲ, Ⅳ(1.6-1.8) than in control group (2.5).Conclusion 1A29 antibody administered either before ischemia or during the 4 h of reperfusion can protect the brain against global cerebral I/R injury.
