Influences of ultraviolet A (UVA) on the secretion and expression of chemokine CXCL11/I-TAC by HaCaT cells
10.3760/cma.j.issn.0412-4030.2010.03.013
- VernacularTitle:UVA辐射对HaCaT细胞分泌和表达趋化因子CXCL11/I-TAC的影响
- Author:
Shiling GENG
;
Shijun SHAN
;
Tongwei ZHANG
;
Jian WU
;
Zhihua WANG
;
Ting XIAO
;
Chundi HE
;
Hongduo CHEN
- Publication Type:Journal Article
- Keywords:
Ultraviolet rays;
HaCaT cells;
Chemokines,CXC
- From:
Chinese Journal of Dermatology
2010;43(3):181-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influences of UVA on the secretion and expression of chemokine CXCL11/I-TAC by HaCaT cells induced by interferon γ (IFN-γ) and tumor necrosis factor α (TNF-α). Methods HaCaT cells were cultured in the presence of IFN-7 and TNF-a and irradiated with UVA of 2, 4 and 8 J/cm~2, respectively; those cells receiving neither treatment with IFN-γ or TNF-α nor UVA irradiation served as the negative control, and those receiving only cytokine treatment but no irradiation as the positive control. After another 24-hour culture, enzyme-linked immunosorbent assay (ELISA) was performed to detect the protein levels of CXCL11/I-TAC in the supernatant of HaCaT celb, real time PCR to measure the mRNA expression of CXCL11/I-TAC in these HaCaT cells. Results As far as the negative control HaCaT cells were concerned, there was a minor secretion of CXCL11/I-TAC protein and expression of CXCL11/I-TAC mRNA. After treatment with IFN-7 and TNF-a of 10 μg/L, the protein and mRNA expressions of CXCL11/ I-TAC were synergistically upregulated, whereas the induced secretion and expression of CXCL11/I-TAC by HaCaT cells were dose-dependently inhibited by UVA irradiation. Conclusions UVA irradiation inhibits the secretion and expression of CXCL11/I-TAC by HaCaT cells, which in turn suppresses the chemotaxis of Th1/ Tel cells in some degree.
