LPS-Induced Migration of Peritoneal B-1 Cells is Associated with Upregulation of CXCR4 and Increased Migratory Sensitivity to CXCL12.
10.3346/jkms.2012.27.1.27
- Author:
Hana MOON
1
;
Jae Ghi LEE
;
Sang Hyuck SHIN
;
Tae Jin KIM
Author Information
1. Division of Pathology, Department of Molecular Cell Biology and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Korea. tjkim@skku.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
B Lymphocyte Subsets;
Chemokine CXCL12;
Chemokine CXCL13;
Chemotaxis;
Receptor, CXCR4;
Lipopolysaccharides
- MeSH:
Adjuvants, Immunologic/pharmacology;
Animals;
B-Lymphocytes/cytology/*drug effects/immunology;
Cell Movement;
Cells, Cultured;
Chemokine CXCL12/metabolism/*pharmacology;
Chemokine CXCL13/metabolism/pharmacology;
Lipopolysaccharides/*pharmacology;
Mice;
Mice, Inbred C57BL;
Peritoneal Cavity/cytology;
Receptors, CXCR4/*metabolism;
Up-Regulation
- From:Journal of Korean Medical Science
2012;27(1):27-35
- CountryRepublic of Korea
- Language:English
-
Abstract:
B-1 cells, which constitute a predominant lymphocyte subset in serosal cavities and produce most of natural antibodies, are subdivided into the CD5+ B-1a and CD5- B-1b cell subpopulations, but the differential roles of B-1a and B-1b cells are not well understood. We report that B-1a cells preferentially migrate out of the peritoneal cavity and upregulate the expression of CXCR4 with heightened sensitivity to CXCL12 and CXCL13 upon LPS treatment compared to B-1b and B-2 cells. Whereas B-1a cells were slightly more abundant than B-1b and B-2 cells in the homeostatic condition, the number of B-1a cells preferentially decreased 48 hr after LPS treatment. The decrease in the peritoneal B-1a cell number was accompanied with increased migration of B-1a cells toward CXCL-12 and CXCL-13 in in vitro transmigration assay using peritoneal B cells from LPS treated mice. The expression level of CXCR4, but not of CXCR5, was also more prominently increased in B-1a cells upon LPS stimulation. LPS-stimulated B-1a cells did not accumulate in omental milky spots in contrast to B-2 cells. These results suggest that B-1a cells actively migrate out of the peritoneal cavity through the regulation of the migratory responsiveness to chemokines and actively participate in systemic immune responses.
