Effect of down-regulation of pituitary tumor-transforming gene (PTTG) on the proliferation and migration of cutaneous squamous cell carcinoma cell line SCL-1
10.3760/cma.j.issn.0412-4030.2010.03.010
- VernacularTitle:下调垂体瘤转化基因对皮肤鳞状细胞癌细胞SCL-1细胞增殖及迁移能力的影响
- Author:
Dong LIU
;
Yonghua XIA
;
Min LI
;
Zhanguo LI
;
Dandan FU
;
Zhongwei TIAN
- Publication Type:Journal Article
- Keywords:
Cell line,tumor,Carcinoma,squamous cell;
Pituitary tumor-transforming gene;
RNA,small interfering;
Cell proliferation;
Neoplasm invasiveness
- From:
Chinese Journal of Dermatology
2010;43(3):174-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of down-regulation of PTTG on the proliferation and migration of cutaneous squamous cell carcinoma cell line SCL-1 and its related mechanism. Methods SCL-1 cells were transfected with control siRNA or PTTG-targeting siRNA (PTTG-siRNA), or remained untransfected. After additional culture, the proliferation of SCL-1 cells as observed with cell counting kit-8 (CCK-8), and cell migration with Boyden chamber. Real-time PCR and Western blot were performed to detect the expression of matrix metalloproteinase 2 (MMP-2), MMP-9 and PTTG. Results The proliferation of SCL-1 cells transfected with PTTG-siRNA was markedly deccelarated in comparision with that of untransfected cells and those transfected with control siRNA (both P< 0.05). Real-time PCR and Western blot disclosed a significant decrease in the mRNA and protein expression of MMP-2, MMP-9 and PTTG in PTTG-siRNA-transfected SCL-1 cells compared with the other two groups of cells. As real-time PCR showed, the expressions of MMP-2, MMP-9 and PTTG in PTTG-siRNA-transfected SCL-1 cells were 0.8%, 23.2% and 21.3% of those in untransfected cells, respectively. Further more, the number of SCL-1 cells migrating through microporous membrane in the Boyden chamber was significantly smaller in PTTG-siRNA-transfected group than in untransfected group and control siRNA-trans-fected group (51.38 ± 4.71 vs 131.33 ± 6.12 and 127.72 ± 5.20, both P< 0.05). Conclusion The down-regulation of PTTG may deccelarate the proliferation and migration of SCL-1 cells and inhibit the expression of MMP-2 and MMP-9 in SCL-1 cells.
