The establishment of an athymic mouse model bearing breast cancer monitored by an in vivo optical imaging system
10.3760/cma.j.issn.1007-631X.2010.03.020
- VernacularTitle:应用活体内光学成像技术观察裸鼠乳腺癌模型的建立
- Author:
Wei ZHU
;
Xinyu QIN
;
Hongwei ZHANG
;
Junxue CHEN
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Disease model,animal;
Luciferases;
Optical imag in vivo
- From:
Chinese Journal of General Surgery
2010;25(3):239-242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the athrmic inouse model of breast cancer in normal position and imitated metastatic breast cancer. Methods Breast cancer cells MDA-MB-231-luc carrying luciferase gene was injected into the athymie mice.The optieal imaging in vivo system was used to observe the establislament of the model. Reseults The breast tumor emerged after we planted the MDA-MB-231-lue cells in the mammary gland fatpads,the volume and photon of the tumor increased during the second weekto the fifth week.After injection by the tail vein,the tumors mainly located in the lungs While after infusion in the left alrtrium.the tumolrs metastate to all over the body. Conclusions We succeeded in the establishment of the athymic mice model of breast cancer.in situ and imitated metastatic breast cancer by iniection into the vena caudalis and the left alttrium.The optical imaging in vivo system could distinctly show the formation of the tumors.
