An experimental study on protective effect of carbon monoxide releasing molecule on severe acute pancreatitis induced lung injury
10.3760/cma.j.issn.1007-8118.2010.03.012
- VernacularTitle:一氧化碳释放分子对重症急性胰腺炎肺损伤保护作用的实验研究
- Author:
Ping CHEN
;
Wenwen WANG
;
Gang WANG
;
Hua CHEN
;
Bei SUN
;
Hongchi JIANG
- Publication Type:Journal Article
- Keywords:
Pancreatitis;
Carbon monoxide releasing molecule;
Lung injury;
Cytokine in-duced neutrophil chemoattractant;
Intercellular adhesion molecule-1
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(3):196-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of carbon monoxide releasing molecule (CORM-2) on severe acute pancreatitis (SAP) induced lung injury in rats.Methods Thirty male Wistar rats were randomly divided into three groups (n= 10) including sham group, SAP group and CORM-2 group.The model of SAP was induced by retrograde infusion of 3.5% sodium taurocholate into the pancreatobiliary duct.CORM-2 (8 mg/kg) was infused through the dorsal artery of penis 0.5 h after establishing the model of SAP in CORM-2 group.All animals were sacrificed 6 h after SAP in-duction.The serum level of tumor necrosis factor-α (TNF-α), pulmonary cytokine induced neutrophil chemoattractant (CINC), intercellular adhesion molecule-1 (ICAM-1) mRNA expression as well as the activity of myeloperoxidase (MPO) in the lung were examined in each group.The wet/dry ratio of the lung was also determined.The lung was scored on the basis of pathological changes.Results Compared with SAP group, pulmonary over-expression of CINC and ICAM-1 mRNA was obviously inhibited in CORM-2 group and the serum TNF-α level, the MPO activity in lung tissue, the wet/dry ratio of lung, and the pathological score of lung injury were significantly lower (P＜0.05).Conclusion Administration of CORM-2 can remarkably reduce the severity of SAP induced lung injury through in-hibiting the over-expression of CINC and ICAM-1 mRNA, down-regulating the serum TNF-α level and subsequently decreasing pulmonary neutrophil infiltration.
