Eicosapentaenoate inhibits palmitic acid induced apoptosis in INS-1

VernacularTitle:Eicosapentaenoate对软脂酸诱导细胞株INS-1凋亡的保护作用研究
Author: Huasheng LIANG ; Yuhua ZHONG ; Huixuan SU
Publication Type:Journal Article
Keywords: Eicosapentaenoic acid/PD; Adenoma,islet cells/DT; Apoptosis/DE; Palmiticacids/AE
From: Journal of Chinese Physician 2010;12(2):162-164
CountryChina
Language:Chinese
Abstract: Objective To study whether eicosapentaenoate can decrease the apoptosis effects in-duced by palmitic acid in INS-1 or not. Methods Based on different condition, there were four groups in this study, including control group , EPA group, palmitic acid group, combination of EPA and palmitic acid group. The grow curve was detected by MTT, and the expressions of bax were detected by Western blot.Cell apoptosis was detected by caspase-3. ROS was detected by CM2H2DCFDA kit after 48h. Result The grow curve of combination of EPA and palmitic acid group was higher than that in plamitic group[24 h :(37.33±1.15)OD vs (30. 79 ± 1.55 )OD, P < 0. 01 ;48 h:(31.50 ± 1.56)OD vs (23.94 ± 1.10)OD, P<0. 01] . Caspase-3 activity and ROS and the expression of bax and SREBP1c in combination of palmitic acid and T0901317 group were lower than those in palmitic acid group[(3566. 67 ± 305.51 )OD vs (4233. 33 ±416. 33)OD]. Conclusion EPA can inhibit apoptosis in INS-1 induced by palmitic acid.