Clinical value of thyroid-stimulating antibody measurement using CHO-hTSHR cell line
10.3760/cma.j.issn.1008-1372.2010.02.008
- VernacularTitle:用CHO-hTSHR细胞检测甲状腺刺激性抗体的研究
- Author:
Yaqin ZHOU
;
Hongmei ZHANG
;
Yan DONG
;
Qing SU
- Publication Type:Journal Article
- Keywords:
Immunoglobalins,thyroid-stimulating/AN;
Ovary/CY;
Hamsters
- From:
Journal of Chinese Physician
2010;12(2):169-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical value of CHO-hTSHR cells in detecting thyroid stimulating antibody (TSAb). Methods The cAMP production and TSAb activity were measured and cal-culated by stimulating CHO-hTSHR cell line with IgGs of normal control group and Graves" disease ( GD )group. TSAb positive standard was set to more than the mean + 2SD of TSAb activities in control subjects.The positive percentage of TSAb activity in GD group was calculated. Results The cAMP production and TSAb activities of GD group were higher than those of normal group[( 353. 65±126. 34 ) pmol/L vs (237.21±77. 15)pmol/L, ( 149. 08±53. 26)% vs ( 100±32. 52)%, P <0. 05] . The value that higher than 165% was set to be positive for TSAb. The positive percentage of TSAb in GD group was 50% ( 14/28). Conclusion CHO-hTSHR cell line constructed by our group is suitable for detecting TSAb activity in the sera of patients with GD.
