Expression of pancreatic ATP-sensitive K~+ channels in rats with chronic pancreatitis and drug interventions
10.3760/cma.j.issn.1007-8118.2010.03.014
- VernacularTitle:慢性胰腺炎大鼠胰腺K_(ATP)通道表达及药物干预研究
- Author:
Quan LIANG
;
Chengrui XUE
;
Xiaolei ZHOU
- Publication Type:Journal Article
- Keywords:
Pancreatitis;
K_(ATP) channel;
Nateglinide
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(3):200-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of pancreatic K_(ATP) channels (Kir6.2/SUR1) in rats with chronic pancreatitis and explore the intervention of nateglinide on the changes.Methods Wistar rats were induced to suffer from chronic pancreatitis and then randomized into model group, nateglinide group and control group.Then OGTT of them were observed.RT-PCR was used to detect the expression of Kir6.2 and SUR1 mRNA, and western blot to detect the expression of Kir6.2 and SUR1 proteins.Results Model rats displayed impaired glucose tolerance (IGT).The expression of Kir6.2 and SUR1 in model group decreased significantly(P＜0.05), and nateglinide displayed up-reg-ulation to the expression in some degree.Conclusion The expression of pancreatic K_(ATP) channels in rats with chronic pancreatitis diminished, which might be the important mechanism of the development of pancreatogenic diabetes.Nateglinide can up-regulate the expression in some degree, which indicates that it may have latent effect of ameliorating the prognosis of patients with chronic pancreatitis.
