Dynamic changes of the expressions of neuroserpin and tissue plasminogen activator during hypoxia/reoxygenation in primarily cultured rat cortical neurons
10.3760/cma.j.issn.1673-4165.2010.02.006
- VernacularTitle:原代培养的大鼠皮质神经元缺氧复氧时神经源性丝氨酸蛋白酶抑制物和组织型纤溶酶原激活物表达的动态变化
- Author:
Xuelian YANG
;
Qiang DONG
;
Liang WANG
;
Ling LIU
- Publication Type:Journal Article
- Keywords:
Neuropeptides;
Tissue plasminogen activator;
Hypoxia-ischemia,brain;
Reperfusion injury;
Neurons;
Rats
- From:
International Journal of Cerebrovascular Diseases
2010;18(2):107-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the ambulatory changes of the expressions of neuroserpin (NSP) and tissue plasminogen activator (tPA) during hypoxia/reoxygenation (H/R) in primarily cultured rat cortical neurons. Methods Cortical neurons were cultured from SD rats within 24 h after birth, and a H/R model was built. The immunofluorescence double staining and Western blot were used to detect the NSP expression in the cultured neurons. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of tPA. Results After oxygen-glucose deprivation (OGD) for 1.5 h, the expression of NSP protein was mildly up-regulated, and it reached the peak at 6 h after reoxygenation (P < 0. 05). Then it decreased slowly and basically restored to the level before hypoxia at 24 h after reoxygenation. The cells expressed a small amount of tPA at 1.5 h after OGD (P < 0. 05 ). With the extension of time for reoxygenation, the tPA content increased gradually. OGD was significantly higher at 6 h after reoxygenation than before (P < 0.01 ). Then it decreased gradually, and it basically restored to the level before reoxygenation at 24 h after reoxygenation. Conclusions The expressions of NSP and tPA Were up-regulated significantly during neuron H/R injury, and the ambulatory changes of NSP and tPA were basically the same.
