Effects of PCNA antisense oligonucleotide and VEGF gene transduction on restenosis after angioplasty
10.3760/cma.j.issn.1007-631X.2010.03.018
- VernacularTitle:PCNA反义寡核苷酸与VEGF防治血管成形术后再狭窄的实验研究
- Author:
Xianqi ZHAO
;
Tiemin ZHANG
;
Bing WANG
;
Yuchuan YANG
;
Chunying SHI
;
Henglu XIANG
;
Hongchi JIANG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factors;
Oligonucleotides,antisense;
Proliferating cell nuclear antigen;
Angioplasty;
Disease modols,animal
- From:
Chinese Journal of General Surgery
2010;25(3):231-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antisense oligonucleofide(PCNA ASON)and vascuiar endothelial growth factor (VEGF) gene traneduction on restenosis after balloon angiopasty.Memods chinese rabbits were randomly divided into control group(I),PCNA ASON(II),VEGF only (Ⅲ),PCNA ASON+VEGF(Ⅳ)groups.Each group included 7 rabbits.Restenosis wasevaluated by pathology immunohistochemistry and Western-blotting for the expression of PCNA,and the depth and area oftunica media and tunicca intima were measured. Results All rabbits experienced restenosis on different severities,especially in control group.Lesions were less severe in PCNA ASON and VEGF groups than in controls.The proliferation of smooth muscle and intima significantly ameliorated in PCNA ASON and VEGF combination group that in PCNA ASON or VEGF groups(P<0.01).But the difference between the PCNA ASON and VEGF group was not significant(P>0.7). Conclusion PCNA ASON and VEGF gene transductionn are effective in preventing restenosis after balloon angioplasty caused vessel injury in rabbits.
