Inducing effect of clodronate-liposome on apoptosis of alveolar macrophages in rats with acute nectotizing pancreatitis
10.3760/cma.j.issn.1007-8118.2010.03.018
- VernacularTitle:氯磷酸二钠脂质体对胰腺炎大鼠肺泡巨噬细胞凋亡的影响
- Author:
Jianxin ZHANG
;
Shengchun DANG
;
Lei CUI
;
Jianguo QU
;
Xuqing WANG
;
Jiangtao YIN
- Publication Type:Journal Article
- Keywords:
Pancreatitis;
Clodronate-liposome;
AM;
Apoptosis
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(3):212-215
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of elodronate-liposome on inducing apoptosis of alve-olar macrophages from rats with acute neetotizing pancreatitis (ANP).Methods The AMs of eight rats with ANP were isolated, purified then incubated from broehoalveolar lavage by the differing rates of attachment of the various cell types in a forty-well cell culture plate.Then they were randomized in-to five groups including control group,blank liposome group( 50 μ1, 100 μ1),clodronate-liposome group (50μ1,100μ1).Values of OD were determined by MTT.AO fluorescence and haematoxylin dye were employed to determine the apoptosis of the AMs.Results There were no significant differences be-tween control group and blank liposome group(50 μ1, 100 μ1).Significant differences were found be-tween control group and clodronate-liposome group(50 μ1, 100 μ1).There were no marked differences between blank liposome group(50μ1, 100 μ1)and clodronate-liposome group(50 μ1,100μ1).AO fluo-rescence and haematoxylin dye were available to define the apoptosis of the AMs.Conclusion Clodr-onate-liposome can effectively induce the apoptosis of the AMs.
