The effect of cinobufagin on the expression of survivin of HHUA cells in endometrial carcinoma
10.3760/cma.j.issn.1673-4246.2010.02.002
- VernacularTitle:华蟾素对子宫内膜癌HHUA细胞存活素表达的影响
- Author:
Shaoguang WANG
;
Xueqiang JIANG
- Publication Type:Journal Article
- Keywords:
Cinobufagin;
Endometrialcarcinoma;
Survivin;
Apoptosis
- From:
International Journal of Traditional Chinese Medicine
2010;32(2):103-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and significance of cinobufagin on the expression of survivin of HHUA cells in the endometrial carcinoma cultured in vitro. Methods HHUA cells of endometrial carcinoma were cultured in vitro. After being intervened by cinobufagin with different concentration, the expression of survivin was measured by RT-PCR and the cellular apoptosis was tested by flow cytometry. Results In the control group: mRNA expression of survivin of HHUA cells in endometrial carcinoma was (1.22±0.23), cellular apoptutic rate was (2.31± 0.98)%; cinobufagin of 0.25 mg/ml didn't affect the survivin and cellular apoptotic rate (q=2.89, P>0.05: q=3.12, P>0.05) ; after being exposed to einobufagin of 2.5 mg/ml, the expression of survivin was (0.73± 0.26), and cellular apoptotic rate was (26.50± 6.36) %, with all of these data being different from the control group significantly (q=5.68, P<0.05; q= 10.23, P<0.05) ; the actions of cinobufagin of 25 mg/ml on mRNA expression of survivin and apoptotic rate were similar to cinobufagin of 2.5 mg/ml (q=3.49, P>0.05; q=4.35, P>0.05) . Conclusion Cinobufagin of 2.5 mg/ml inhibit the mRNA expression of survivin and proliferation of HHUA cells in endometrial carcinoma.
