Preparation of hematoporphyrin-loaded PLGA ultrasound microbubble and optimization of formulation
10.3760/cma.j.issn.1004-4477.2010.03.027
- VernacularTitle:载血卟啉的乳酸/羟基乙酸共聚物超声微泡造影剂制备及工艺优化
- Author:
Yaping ZHANG
;
Haitao RAN
;
Zhigang WANG
;
Hui ZHANG
;
Meng AO
;
Yefeng ZHU
- Publication Type:Journal Article
- Keywords:
Microbubbles;
Hematoporphyrins;
Technology,medical
- From:
Chinese Journal of Ultrasonography
2010;19(3):258-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the formulation of a new kind of ultrasound contrast agents carrying the sensitizer of hematoporphyrin(HP)with[Poly(lactic-co-glycolic acid),PLGA]for material.Methods The technique of double emulsion was applied to produce HP loaded PLGA ultrasound microbubbles,which was optimized through orthogonal test using encapsulation efficiency for the detected index.Then morphology and distribution of HP-PLGA microbubbles were observed through light microscope and scaning electron microscope.The size,Zeta potential and the properties of releasing behavior and ultrasound imaging in vitro of Hp-PLGA contrast agents were detected.Results The optimization parameters were picked out as 10 mg/ml for concentration of HP,40 mg for PLGA,and 1/5 for volume ratio of water inside to dichloromethane.The optimized HP-PLGA contrast agents were spheric with the mean size of 602.3 nm,and Zeta potentiaI of-(17.1±1.6)mV.The drug loading and encapsulation efficiency of HP-PLGA were(2.15±0.15)%and(63.5±2.6)%,respectively.And the releasing behavior of HP-PLGA contrast agents in vitro was that after an obvious release of about 35.1%in former 24 h,there were 86.5%HP-PLGA released within 14 days.The ultrasound imaging of HP-PLGA could be enhanced obviously in vitro.Conclusions The self-made HP-PLGA ultrasound microbubble might be a useful tool for delivering sensitizer and thus provide a novel strategy for sonodynamic therapy on tumor.
