Protective effect of tissue-engineered artificial nerve on peripheral target organ and spinal cord neurons after rat sciatic nerve defect
10.3760/cma.j.issn.1001-8050.2010.03.023
- VernacularTitle:大鼠坐骨神经缺损后组织工程人工神经对外周靶器官及脊髓神经元的保护作用
- Author:
Hua YOU
;
Shusheng JIAO
;
Shuainan FENG
;
Jianmei CHEN
;
Bingcang LI
- Publication Type:Journal Article
- Keywords:
Wounds and injures;
Peripheral nerves;
Tissue engineering
- From:
Chinese Journal of Trauma
2010;26(3):265-269
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of olfactory ensheathing cell(OEC)Schwann cell(SC)-extracellular matrix(ECM)-poly(DL-lactide-co-glycolide acid)(PLGA)bridging complex on peripheral target organ and spinal cord neurons after rat sciatic nerve defect.Methods A 15 mm right sciatic nerve defect model was established in SD rats and repaired with OEC-SC-ECM-PLGA bridging complex that contained OEC,SC,ECM and self-made PLGA conduit.At the same time,the study set OEC-ECM-PLGA group,SC-ECM-PLGA group,ECM-PLGA group,PLGA group and nerve autograft control group.At 1,3,6 and 9 weeks after surgery,the gastrocnemius muscle water weight test and motor end-plate test were performed.At the 9th week after surgery,CM-DiI and horseradish peroxidase(HRP)retrograde tracing were performed.Results The gastrocnemius muscle water weight and number of motor end-plate were decreased in all groups after surgery but gradually increased after three weeks except for ECM-PLGA group and PLGA group.At the 9th week,OEC-SC-ECM-PLGA group showed no statistical differences with nerve autograft group in aspects of gastrocnemius muscle water weight,number of motor end-plate and length of motor end-plate major axis(P ＞ 0.05).At the 9th week,CM-DiI and HRP retrograde tracing found that the number of positive neurons in spinal cord in OEC-SC-ECM-PLGA group showed no statistical differences compared with nerve autograft group(P ＞0.05).Conclusions OEC-SC-ECM-PLGA bridging complex can partially protect peripheral target organ and spinal cord neurons after rat sciatic nerve defect.
