Evaluation of repair of large segmental defects with vascularized tissue-engineered bone induced by endothelial progenitor cells in rabbits
10.3760/cma.j.issn.1001-8050.2010.03.025
- VernacularTitle:血管内皮祖细胞促血管化组织工程骨修复兔大段骨缺损的效果评价
- Author:
Xuehui WU
;
Zhao XIE
;
Qingyi HE
;
Jianzhong XU
;
Ling ZENG
;
Weijun CHEN
;
Dong SUN
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Stem cells;
Fracture healing;
Bone defect
- From:
Chinese Journal of Trauma
2010;26(3):275-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the bone healing effect of vascularized tissue-engineered bone induced by endothelial progenitor cells(EPCs)in repair of large segmental radius defects in rabbits.Methods A total of 68 healthy New Zealand white rabbits were enrolled in the study and randomized into three groups,ie,experimental group(EPCs group):EPCs plus bone marrow mesenchymal stem cells (BMSCs)plus decalcified bone matrix(DBM);control group:BMSCs plus DBM;sham control group:pure DBM.Materials mentioned above were implanted into middle radius defects for 15 mm.At 12 and 16 weeks post-operatively,X-ray test,bone mineral density test,histological light microscopic test,osteocalcin immunohistochemical staining test and biomechanical test were carried out.Results Growth and plasticity of callus,speed of medullary cavity recanalization,bone healing speed and biomechanical intensity in the experimental group were all significantly better than those of control group.Conclusions Vascularized tissue-engineered bone induced by EPCs has strong osteegenic ability,can accelerate bone healing and hence is an effective method for repair of large segmental bone defects.
