Experimental study of lipid ultrasound contrast agent with galactose-carrying poly-L-lysine target to HepG 2 cells
10.3760/cma.j.issn.1004-4477.2010.03.026
- VernacularTitle:脂质超声造影剂携半乳糖化多聚赖氨酸靶向结合HepG2细胞的实验研究
- Author:
Jinhong YU
;
Zhigang WANG
;
Zhaoxia WANG
;
Pan LI
;
Haitao RAN
;
Ao LI
;
Meng AO
- Publication Type:Journal Article
- Keywords:
Microbubbles;
Liposomes;
Liver neoplasms
- From:
Chinese Journal of Ultrasonography
2010;19(3):255-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the targeted capacity of ultrasound contrast agent with a galactosepoly-L-lysine ligand,and lay the foundation for targeted imaging in vivo and the treatment of liver cancer.Methods The small molecular targeted ligand was synthetized by a method of reductive amination,the targeted lipid microbubbles were performed by combining lipid microbubbles with the targeted ligand,the targeted effectiveness was observed by fluorescence microscopy.Results Galactose and poly-L-lysine were combined effectively after 24 h with the molar ratio 1:1 00,the average particle size of the targeted lipid microbubbles was 2 micron,the targeted combine of the HepG2 liver cancer cells with the targeted ultrasound contrast agent was observed by laser scanning confoeal microscope.Conclusions The targeted lipid miembubbles can target effectively to the HepG2 cells.
