Pharmacokinetics of levofloxacin in rat pancreas
10.3760/cma.j.issn.1674-1935.2010.02.007
- VernacularTitle:盐酸左氧氟沙星在大鼠胰腺的药代动力学研究
- Author:
Deding LIU
;
Heping XIAO
;
Zhuo WANG
;
Jia ZHOU
;
Bin SONG
;
Lin YANG
;
Rui LIU
;
Yi HUANG
- Publication Type:Journal Article
- Keywords:
Levofloxacin;
Microdialysis;
Pharmacokinetics;
Pharmacodynamics;
High performance liquid chromatography
- From:
Chinese Journal of Pancreatology
2010;10(2):92-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pharmaeokinetics of levofloxacin in rat's pancreatic tissue. Methods Pancreatic tissue and blood were sampled in vivo by microdialysis simultaneously. The concentrations of levofloxacin in beth blood and tissues were measured by high performance liquid chromatography. All date were analyzed by WinNonlin software. Results The maximum concentration of free levofloxacin in blood and pancreatic tissue were (65.23 ± 12.9) μg/ml at 10min and (30.56±3.22) μg/ml at 20 min, respectively, then beth continuously decreased. Concentration of free levofloxacin in pancreatic tissue was higher than that in blood from 20min to 100min, then returned to similar level. The area under the concentration curve(AUC)of unbound levofloxacin was(2465.11±258.56)min·μg~(-1)·ml~(-1) in pancreas,and (2914.38±205.73)min·μg~(-1)·ml~(-1) in blood.Conclusions Microdialysis with reversed phase high performance liquid chromatography established in this essay could be used to determine the pharmacokinetics of levofloxacin objectively. High concentration of levofloxacin in pancreatic tissue and blood was observed.
