An study of Tephroseris Kirilowii Turez.Houlub extract on cell cycle and apoptosis marker AnnexinV/PI of U266
10.3760/cma.j.issn.1673-4246.2010.03.008
- VernacularTitle:狗舌草提取物对多发性骨髓瘤U266细胞周期及凋亡标志物AnnexinV/PI的作用
- Author:
Zhigang MA
;
Huibing FAN
;
Xiaoli FAN
;
Junqing XU
;
Xiaolu ZHANG
- Publication Type:Journal Article
- Keywords:
Tephroseris Kirilowii Turez.Houlub extract;
Multiple myeloma;
Cell evele;
Cell apoptosis marker Annexin V/PI
- From:
International Journal of Traditional Chinese Medicine
2010;32(3):207-208
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study Tephroseris Kirilowii Turez.Houlub extract on cell cycle and apoptosis marker AnnexinV/PI of U266 in vitro..Methods U266 cells were cultured together with Tephroseris Kirilowii Turez.Houlub extract.Cell cycle and apoptosis marker AnnexinV/PI were detected by flow cytometry(FCM).Results After exposure of U266 cells to Tephroseris Kirilowii Turez.Houlub extract.the cell cycle distribution was changed.There Was a decrease of cells in the G0/G1 phase with an increase of cells in the S phase and G2/M phase and apoptosis.FCM with staining of Annexix V FITC/PI showed a dependence of apoptotic cells with the dosage of Tephroseris Kirilowii Turez.Houlub extract.Conclusion Tephroseris Kirilowii Turez extract has strong cell apoptosis effect on U266 cells.
