Fluorescence PCR for detecting ureA gene of Helicobacter pylori in feces
10.3760/cma.j.issn.1674-2397.2010.03.009
- VernacularTitle:荧光PCR技术在粪便幽门螺杆菌ureA基因检测中的应用
- Author:
Shenghai WU
;
Lihui XU
;
Daojun YU
;
Xianjun WANG
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Fluorescence polymerase chain reaction;
Feces;
Diagnosis
- From:
Chinese Journal of Clinical Infectious Diseases
2010;3(3):162-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of fluorescence PCR in detecting ureA gene of Helicobacter pylori(HP)in feces.Methods Fluorescence PCR was used to detect ureA gene of HP in feces from 50 patients,including 23 confirmed by gastric biopsy urease test and histological staining.Bacterium culture and serum antibody detection were also performed, and chi-square test was used to compare the sensitivity,specificity,positive predictive value and negative predictive value among three methods.Results The sensitivity,specificity,positive predictive value and negative predictive value of fluorescence PCR were 1.00,0.96,96.O%and 100.0%,while those for HP culture were 0.78,1.00,100.0%and 84.0%,and thee for serum antibody detection Was 0.96,0.74,76.O%and 95.0%.There were significant differences in sensitivity and negative predictive value between PCR and bacterium culture (X2=5.60 and 4.44,P<0.05),and significant differences in specificity and positive predictive value between PCR and serum antibody detection(X2=5.28 and 4.08,P<0.05).Conclusion ureA gene detection in feces by fluorescence PCR is of value for the diagnosis of HP infection.
