The effect of PAT on PCNA of human colon carcinoma implants in nude mice

VernacularTitle:酒石酸锑钾对人结肠癌裸鼠移植瘤增殖细胞核抗原的影响
Author: Yanguo YANG ; Shaoyong XU ; Shaojun ZHANG ; Yong DU ; Junhua HU
Publication Type:Journal Article
Keywords: Antimony potassium tartrate/PD; Colonic neoplasms/DT; Proliferating cell nuclear antigen/DE/ME
From: Journal of Chinese Physician 2010;12(4):453-455
CountryChina
Language:Chinese
Abstract: Objective To study the potential therapeutic effect of potassium antimonyl tartrate (PAT) on human colon cancer in transplanted tumor nude mice models. MethodsSixty transplanted animal models were constructed with colon cancer cell line SW480 injected in nude mice. Nude mice were then random divided into 4 groups ( n = 15) :Normal saline group, 5-Fu group and different dose of PAT groups [ (20 mg/( Kg · d) ,40 mg/( Kg · d) ]. The volume of mass was measured every 3 days. After final-administration for 24 hours, immunohistochemical staining was used to detect the expression of PCNA in colon cancer cells. ResultsAfter the use of PAT, the growth of mass slowed down. PCNA levels [ (63. 63 ±8. 88)％ ,(59. 13 ±6. 15)％ ,(33. 38 ± 12. 76)％ ] in SW480 cells was reduced by PAT( P <0. 05, P <0. 01 ). ConclusionPAT potentially inhibited the growth of colon cell lines and induced apoptosis of SW480 colon cancer cells.