MDC/CCL22-CCR4 axis in milky spots in peritoneal carcinomatosis of gastric cancer

VernacularTitle:CCL22-CCR4轴在胃癌腹膜乳斑转移的机制研究
Author: Gnng HUANG ; Xiang HU ; Liang CAO ; Hong LUO
Publication Type:Journal Article
Keywords: Stomach neoplasms; Neoplasm metastasis; Chemotactic factors,macrophage; Milky spot
From: Chinese Journal of General Surgery 2010;25(4):322-325
CountryChina
Language:Chinese
Abstract: Objective To study the role of MDC/CCL22-CCR4 axis in milky spots in peritoneal carcinomatosis of gastric cancer.Methods We examined the expression of CCR4 in 5 gastric cancer cell lines by RT-PCR and Western-blot.Cell growth rate of BGC-823 cell lines before and after incubated with CCL22 was detemined by MTT assay.The effects of CCL22 on invasion of BGC-823 cells into Matrigel were measured using Transwell test.Immunohistochemistry was conducted to detect the expression of CCR4.Results CCR4 mRNA and protein expression were detected in all 5 human gastric cancer cell lines:CCL22had a direct promotive effect on BGC-823 cell prolifetation and invasion.There was CCR4 expression in Stomach neoplasms.The expression of CCR4 in primary tumor was correlated with tumor pathological differentiation(P<0.05),but not with age of patients,gender,location of tumor,tumor size,tumor stage,lymph node metastasis(P>0.05).Conclusions The MDC/CCL22-CCR4 axis may be one of the mechanism of peritoneal metastasis in milky spot in gastric cancer.