Expression of Toll-like receptors 3 (TLR3) on peripheral blood mononuclear cells and serum IFN-γ,TNF-α levels in children with acute rotavirus diarrhea
10.3760/cma.j.issn.1673-4912.2010.03.009
- VernacularTitle:急性轮状病毒腹泻患儿外周血单核细胞Toll样受体3 mRNA表达及血清γ干扰素和肿瘤坏死因子α水平
- Author:
Jie ZI
;
Qian WANG
;
Lei ZHENG
;
Yuan ZHAO
;
Lipeng LIN
- Publication Type:Journal Article
- Keywords:
Rotavirus(RV);
Toll like receptor 3;
Peripheral blood mononuclear cells(PBMC);
IFNγ;
TNF-α;
Diarrhea:Children
- From:
Chinese Pediatric Emergency Medicine
2010;17(3):214-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between TLR3 mRNA expression on peripheral blood mononuclear cells(PBMCs)and acute rotavirus(RV)diarrhea.Methods Sixty-one children with acute RV diarrhea served as study subject,the expression of TLR3 mRNA on PBMCs was detected by real-time fluorescence quantitative RT-PCR.the concentrations of IFN-γand TNF-α in serum were measured by the method of Enzyrme-linked immunosorbent assay(EUSA).Results The expression of TLR3 on PBMCs and the serum levels of IFN-γ and TNF-α in the serious diarrhea group were 0. 820±0.051,(33.67±12.88)Pg/ml, (62.21±14.65)pg/ml,respectively,while it were 0.717±0.040,(24.01±10.06)pg/ml,(50.99± 12.18)pg/ml in the slight diarrhea group,and 0.525±0.029,(12.52±5.19)pg/ml,(28.65±7.44)pg/ml in the control group.Compared with the control group.the expression of TLR3 on PBMCs and the serum levels of IFN-γ,TNF-α in the serious and slight diarrhea group were significantly higher(P<0.01).There were significant differences between the serious and slight diarrhea group(P<0.01).There were positive relationship between the expression of TLR3 on PBMCs and tHe serum IFN-γ,TNF-α levels(r=0.431,P< 0.05,r=0.372,P<0.05).Conclusion The expression of TLR3 on PBMCs in children with acute rotavirus dialThea iS up-regulated,TLR3 and its mediated immune response are associated with the development of acute rotavirus diarrhea.
