Anti-proliferative effect of CDC2 kinase antisense on proliferative cholangitis
10.3760/cma.j.issn.1007-631X.2010.05.020
- VernacularTitle:以CDC2激酶为靶点的反义基因治疗大鼠慢性增生性胆管炎
- Author:
Yong ZHOU
;
Fuyu LI
;
Nansheng CHENG
;
Lisheng JIANG
;
Ning LI
;
Quangsheng LI
- Publication Type:Journal Article
- Keywords:
Cholelithiasis;
Cholangitis;
CDC2 protein kinase
- From:
Chinese Journal of General Surgery
2010;25(5):398-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective Proliferative cholangitis (PC) is responsible for stone recurrence and biliary restenosis, this study was to investigate the and-proliferative effect of CDC2 kinase shRNA on PC. Methods The common bile duct of PC rat model was given an intralumenal administration of 0. 5 ml of CDC2 kinase shRNA. Results CDC2 kinase shRNA treatment effectively inhibited the expression of CDC2 kinase,PCNA, and procollagen I , resulting in the inhibition of hyperplasia of biliary epithelium, submucosal gland, and collagen fibers. Also, the lithogenic potentiality of PC decreased due to the inhibition of endogenous β-glucuronidase secretion. Conclusion The anti-proliferative effect of CDC2 kinase shRNA on PC may prevent biliary restenosis and stone recurrence.
