Changes of PKC membrane translocation of the brain in neonatal SD rats after hypoxic-ischemic insult
10.3760/cma.j.issn.1674-6554.2010.04.006
- VernacularTitle:新生儿缺氧缺血性脑病模型鼠脑内蛋白激酶C膜转位的变化
- Author:
Dunchen LI
;
Gang LIU
;
Xianmin YIN
- Publication Type:Journal Article
- Keywords:
Protein kinase C;
Hypoxic-ischemic encephalopathy;
Membrane translocation;
Rat
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2010;19(4):307-308
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of protein kinase(PKC)in the nosogenesis of hypoxic-ischemic encephalopathy(HIE).Methods Twenty-four seven-day-old SD rats were divided randomly into normal group (N),model control group(C)and HIE group(HIE).The biochemistry techniques of SDS-PAGE and western bolt were used to examine the level of PKC membrane translocation of cortex and hippocampi.Results The level of PKC membrane translocation of cortex and hippocampi in N group was 0.28±0.02,0.29±0.02 respectively,and 0.29±0.02,0.30±0.02 in C group,and 0.38±0.04,0.40±0.06 in group HIE.The level of PKC membrane translocation of cortex and hippocampi was higher in HIE group as compared with that in N,C group(P＜0.01),but there was no different between N group and C group(P＞0.05).Conclusion PKC are activated in HIE,which indicates that PKC may involve in the pathophysiologic mechanism of HIE.
