Repair of cartilage defects using chondrocyte and osteoblast composites in vitro cultured in bioreactor
10.3760/cma.j.issn.1001-8050.2010.04.026
- VernacularTitle:应用生物反应器体外培养骨软骨复合体修复犬关节软骨损伤
- Author:
Qiang REN
;
Shui SUN
;
Lei ZHANG
;
Dong WANG
;
Shuai WU
;
Xitao SUN
- Publication Type:Journal Article
- Keywords:
Calcium phosphates;
Bioreactors;
Cartilage defect
- From:
Chinese Journal of Trauma
2010;26(4):361-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the feasibility of chondrocyte and osteoblast composites in vitro cultured in bioreactor in repairing cartilage defects.Methods Marrow mesenchymal stem cells were isolated and cultured in vitro,and then were induced to chondrocytes and osteoblasts by growth factor.Chondrocytes and osteoblasts were cocultured in bioreactor for 21 days to form the composites.The adhesion,extension and proliferation of chondrocytes and osteoblasts were observed under scanning electron microscope.The cartilage defects on canine model were repaired with the chondrocyte and osteoblast composites.Results The induced chondrocytes and osteoblasts had fine adhesion,extension and proliferation in the β-TCP scaffold.The repaired tissues in experimental group maintained their thickness to the full depth of the original tissues.A statistical difference was observed between negative control group and experimental group(q=12.337 0,P ＜ 0.01)and between blank control group and experimental group (q=31.539 3,P ＜0.01).Conclusion Perfusion bioreactor makes chondrocyte and osteoblast survive and proliferate in a three-dimensional scaffold and increases the composition rate of the chondrocyte and osteoblast.
