TIMP-1 inhibitor expression in autologous vein graft
10.3760/cma.j.issn.1007-631X.2010.05.022
- VernacularTitle:金属蛋白酶1组织抑制因子在大鼠自体移植静脉中的表达研究
- Author:
Shaokun ZHANG
;
Bing GUO
;
Yufeng ZHOU
;
Jun NING
;
Chunlai LI
;
Lei WANG
- Publication Type:Journal Article
- Keywords:
Transplantation;
Tissue inhibitor of metalloproteinase-1;
Intimal hyperplasia;
Vein grafts
- From:
Chinese Journal of General Surgery
2010;25(5):405-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of matrix metalloproteinases 1 (TIMP-1)products inhibitor on smooth muscle cell (SMC) proliferation in rat autologous vein graft.Methods Autogenous vein transplantation model was established in 30 Wistar rats. The vein grafts were harvested at different time after grafting. The change of TIMP-1 was detected by using hematoxylin and eosin, immunohistochemistry and in situ hybridization (ISH). Results 1. Changes of histopathology in vein grafts: Intimal hyperplasia (IH) could be seen in 7 - 14 days, the peak at 2 ~ 4 weeks after operation.2. ISH results: TIMP-1mRNA positive cells appeared at 24 hours, increased significantly in 72 hours and reached the peak at 1 ~ 2 week after operation. There was significant difference between day 1 and 2 week.TIMP-I expression was not detected in normal vessels (P <0. 01). 3. Immunohistochemistry results: There was trace TIMP-I expression in normal vessels. The expression of TIMP-1 appeared at 72 hours after vein graft, increased mostly in 1 week, reached the peek at 2 week and reduced later. There was significant difference between day 1 and 2 week (P < 0. 01). Conclusions 1. The activation of TIMP-1 exists in autogenous vein grafts. 2. The intima experienced hyperplasia in spite of increased secretion of endogenous TIMP-1 after autogenous vein grafts.
