The effect of adenovirus-mediated ING4 expression on breast cancer cells
10.3760/cma.j.issn.1007-631X.2010.04.015
- VernacularTitle:Ad-ING4对乳腺癌的生长抑制作用研究
- Author:
Zhengyi LI
;
Weihua SHENG
;
Yufeng XIE
;
Huicui YANG
;
Jicheng YANG
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Genes,tumor suppressor;
Apoptosis;
ING4
- From:
Chinese Journal of General Surgery
2010;25(4):313-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of inhibitor 4 of growth(ING4)delivered by adenovirus on human breast carcinoma cell MDA-MB-231.Methods MDA-MB-231 human breast carcinoma cells were irfected with Ad-ING4.The expression level of ING4 gene was detected by RT-PCR and Western blot;The growth inhibition,cell-cycle alteration,and apoptosis were detected by MTT,Flowcytometry and Hochest33258 staining.respectively.RT-PCR was used to detect the transcription of Bax,Bc1-2,Survivin genes;The expression level of Ang-1 gene was detected by ELISA;Ad-ING4 was given intratumorally in athymic nude mice beating MDA-MB.231 tumors.and then tumor growth was monitored;The expression of Bc1-2,Bax and Caspase-3 was analyzed by immumohistochemistry.Results ING4 was successfully transcribed and translated iU MDA-MB-231 cells:Ad-ING4 significantly inhibited the proliferation and induced G_2/M phase arrest to(24.86±1.24)% and cell apoptosis of MDAMB-231.Intratumoral injection of Ad-ING4 suppressed the tumor growth obviously with a inhibitory rate of 49%;Immumohistochemistry showed that the expression of Bax,Caspase-3 were up-regulated and the expression of Bc1-2.Survivin,CD34 were down-regulated by Ad-ING4.Conclusions Ad-ING4 can inhibit the growth of MDA-MB-231 cells and induce apoptosis in vitro and in vivo.
