The diagnostic value of fibro-optic bronchoscopy examination combined with detection of aberrant p16 promoter methylation in lung cancer

VernacularTitle:纤维支气管镜联合p16基因甲基化检测对肺癌的诊断价值
Author: Zhuojun HU ; Daying LIU ; Hongbo HU ; Yiping CHEN ; Jinping CHEN ; Hongchun TAN ; Jingang ZHOU ; Hailiang LIU ; Yan ZHONG
Publication Type:Journal Article
Keywords: Bronchoscope; Gene,p16
From: Chinese Journal of General Practitioners 2010;9(5):349-351
CountryChina
Language:Chinese
Abstract: Fibro-optic bronchoscopy (FB) examinations were undertaken in 42 cases with lung cancer and 25 cases with benign lung disease; methylation-specific PCR was performed in plasma, sputum and bronchoalveolar lavage fluid (BALF) specimen for detection of p16 gene promoter methylation in all patients.Of the 42 cases of lung cancer, the positive rates of p16 gene promoter methylation were 59.5% in BALF, 52.4% in plasma and 47.6% in sputum, respectively; while p16 gene promoter methylation was detected only in one plasma sample from 25 cases with benign lung disease ( P ＜ 0.05 ).The sensitivity,specificity and overall accuracy of FB were 59.5%, 100.0% and 74.6%, respectively.The sensitivity,specificity and overall accuracy of FB combined with aberrant p16 gene methylation in diagnosis of lung cancer were 92.9%, 96.2% and 94.0%, respectively.The FB examinations combined with detection of aberrant p16 gene methylation can further improve the accuracy to diagnosis of lung cancer.