Transfection of GFP gene in rat C6 glioma cells enhanced by ultrasound-mediated microbubble destruction
10.3760/cma.j.issn.1004-4477.2010.05.030
- VernacularTitle:超声联合声诺维辐照增强GFP基因在鼠胶质瘤C6细胞中的转染
- Author:
Changjun WU
;
Junfeng WANG
;
Qianyi QIU
;
Miao ZHENG
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Glioma;
Transfection
- From:Chinese Journal of Ultrasonography
2010;19(5):443-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether ultrasound (US) exposure combined with microbubble destruction could be used to enhance non-viral gene delivery in rat C6 glioma cells. Methods Microbubbles were prepared and gently mixed with plasmid DNA. The mixture of the DNA and microbubbles was administered to cultured C6 cells under different US/microbubble conditions. US parameters adopted in this study were frequency 1 MHz, output intensity 1 W/cm2, duty cycle 20%, exposure time 30 seconds. Transfection efficiency and cell viability were assessed by FACS analysis, confocal laser scanning microscopy, and Try pan blue staining. Results Microbubble with US exposure could significantly enhance the reporter gene expression as compared with other groups. No statistical significant difference was observed in the glioma cell viability between different groups. Conclusions US-mediated microbubble destruction has the potential to promote safe and efficient gene transfer into C6 cells,and it may be useful for safe clinical gene therapy of brain cancer without a viral vector system.
