Study on expression of P16, CD44v6 and TGF-β1 in gastric carcinoma and their relationship
10.3760/cma.j.issn.1008-1372.2010.05.015
- VernacularTitle:P16、CD44v6和TGF-β1在胃癌中的表达及其相关性研究
- Author:
Hongming SUN
;
Wei GONG
- Publication Type:Journal Article
- Keywords:
Genes,pl6/ME;
Transforming growth factor betal/ME;
Stomach neoplasms/ME
- From:
Journal of Chinese Physician
2010;12(5):615-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of P16, TGF-β1 and CD44v6 in gastric carcinoma and the relationship between their expression and clinicopathological features. Methods The expressions of P16, TGF-β1 and CD44v6 in 78 cases of gastric carcinoma, 20 cases gastric epithelial dysplasia and 25 cases normal gastric tissues were detected by immunohistochemistiy (SP method). Results The expressions of P16 in gastric carcinoma (46. 2% ) and gastric epithelial dysplasia (60. 0% ) were markedly lower than that in normal gastric tissues (92. 0% ). The expressions of CD44v6 in gastric carcinoma (51.3%) and gastric epithelial dysplasia (45.0% ) were markedly higher than that in normal gastric tissues (4.0% ). The expression of TGF-β1 in gastric carcinoma (65.4% ) was much higher than the counterparts in normal gastric group (12. 0% ) and gastric epithelial dysplasia group (35.0% ). P16 was positively correlated with the differentiation degree of gastric carcinoma, the depth of invasion, lymphatic metastasis and TNM stage ( P < 0. 05 ), and it had no significant correlation with tumor size,vessel tumor thrombus and the progression of the disease. CD44v6 was positively related with the invasive depth of gastric carcinoma, lymphatic metastasis, progression and TNM stage ( P <0.05) and had no significant correlation with differentiation degree of tissue, tumor size and vessel tumor thrombus ( P >0.05). TGF-β1l was positively correlated with the differentiation degree of gastric carcinoma, the depth of invasion, lymphatic metastasis and TNM stage ( P < 0.05 ) and had no significant correlation with tumor size, vessel tumor thrombusand the progression of the disease. There was a significant positive correlation between the expressions of CD44v6 and TGF-β1 of on gastric carcinoma ( r = 0. 532, P < 0.05 ). And a negative correlation was found between CD44v6 and P16 ( r = - 0. 615, P < 0. 05 ). Conclusions P16 ,TGF-β1 and CD44v6 may play a role in the tumorigenesis and progression of gastric carcinoma in some degree. Combined detection of P16, TGF-β1 and CD44v6 may be helpful to judge the degree of malignancy and potential lymph node metastasis and evaluate the prognosis.
