The experimental study of slow-release microcapsules of hepatocyte growth factor on angiogenesis in infracted rabbit myocardium
10.3760/cma.j.issn.1008-1372.2010.05.005
- VernacularTitle:肝细胞生长因子缓释微胶囊诱导缺血心肌血管新生的实验研究
- Author:
Hui WANG
;
Hua CHEN
;
Mingrui LV
;
Zuhui TANG
;
Zhaorui ZHANG
;
Zhilong ZHANG
;
Yong LIU
;
Fan NIU
;
Xinmin ZENG
- Publication Type:Journal Article
- Keywords:
Hepatocyte growth factor/PD;
Myocardial ischemia/DT;
Neovascularization,patho-logic/DT;
Delayed-action preparations
- From:
Journal of Chinese Physician
2010;12(5):588-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of slow-release microcapsules of HCF( hepatocyte growth factor) on angiogenesis in infracted myocardium.Method Myocardial infarction was induced in 30 New Zealand rabbits by ligating the middle of left descending coronary artery. Group Ⅰ ( n = 10) was served as a control group, group Ⅱ ( n =10) as a blank microcapsule group, group Ⅲ ( n = 10) as experimental group with each microcapsule contains 1 μgHGF as HCF group. In group Ⅱ andⅢ, 5 blank microcapsules or FGF slow-release microcapsules were implanted into myocardium under epicedium between the left descending coronary artery and left circumflex branch. The heart function of each rabbit was evaluated with echocar-diography and cheterization, angiogenesis was evaluated by immunohistochemical technique 6 weeks later.Result As compared with group Ⅰ and Ⅱ , rabbits treated with HGF had higher microvessel counts ( P < 0. 01), and LVFS and EF were significantly increased [ (101. 28±19. 50,105. 28 ±18. 28,161. 28 ±15. 85, P <0.01 ]. Conclusion Subepicardial implantation of HGF slow release microcapsule in the infracted rabbit model can enhance effective angiogenesis and improve left ventricular function.
