Inhibition of miR-375 decreases lipoapoptosis in NIT-1 β cells

VernacularTitle:抑制miR-375降低NIT-1胰岛β细胞脂性凋亡
Author: Xuejuan XU ; Yan LI ; Shanying LIU ; Ying LIANG ; Li YAN ; Zuzhi FU
Publication Type:Journal Article
Keywords: RNA interference; Beta cell apoptosis; MiR-375; Myotrophin; 2'-O-me-375
From: Chinese Journal of Endocrinology and Metabolism 2010;26(5):402-405
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of miR-375 inhibited by 2'-O-me-375 on lipoapoptosis of NIT-1 pancreatic β cells. Methods NIT-1 cells were divided and treated according to the optimal condition: mock (without lipofectamine) ,lipofectamine( transfected only with lipofectamine) ,NC-miRNA (transfected with negative control miRNA) ,and 2'-O-me-375( transfected with 2'-O-me-375) groups. 72 hours later, all cells in each group were cultured with 500 μmol/L palmitate for 48 h. The percentage of apoptotic cells was detected by Hochest33342 staining and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL). The protein expression of myotrophin ( V1 ) , a target gene of miR-375, was detected by Western blotting. Results Compared to the other three groups,the cell apoptosis rate of 2'-O-me-375 group was the lowest (P<0.01) .along with the highest VI expression level(P<0. 01). Conclusion Inhibition of miR-375 decreases pancreatic (3-cell lipoapoptosis.