Contrast Observation on Protective and Therapeutic Effects of Different Therapies and Formulas on Myocardial Ischemia Reperfusion Injury in Rats
10.3760/cma.j.issn.1673-4246.2009.06.001
- VernacularTitle:不同治法方药对心肌缺血再灌注损伤大鼠防治作用的对比研究
- Author:
Danli TANG
;
Huamin ZHANG
;
Mingjie SUN
;
Yu SUI
- Publication Type:Journal Article
- Keywords:
Myocardial ischemia-reperfusion injury;
Treating both phlegm and stasis
- From:
International Journal of Traditional Chinese Medicine
2009;31(6):485-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of different Chinese medical treatments on myocardial ischemia-reperfusion injury in rats. Methods 60 SD rats were divided into 5 groups randomly: a sham-operated group, a model group, a removing phlegm and enlarging chest group, an activating blood and dissolving stasis group, and a treating both phlegm and blood stasis group. The model of MI/RI of the myocardium was reproduced by ligation of left descending artery for 30min followed by releasing the ligation for 2 hours in rats. Serum contents of LDH-L, CK were measured , TNF-αand ICAM-1 expressions in myocardium were determined with immunohistochemistry and myocardial ultrastructure at the ischemia region was observed with the transmission electron microscope after myocardial reperfusion injury. Results Compared with the model group, LDH-LXK and TNF-αICAM-1 levels were lower, myocardial ultrastructural changes were improved in all the other four groups treated by different Chinese medicine (P<0.01 or P<0.05), especially in the group treating both phlegm and stasis. Conclusion The removing phlegm and enlarging chest method, activating blood and dissolving stasis method, treating both phlegm and blood stasis method can protect myocardium from M1/R1, especially the method of treating both phlegm and blood stasis.
