Influence of epidermal growth factor on proliferation, migration and apoptosis of adipose-derived stem cells in vitro

VernacularTitle:表皮生长因子对脂肪干细胞体外增殖、迁移和凋亡的影响
Author: Lei ZHANG ; Yang XIAO ; Zhendong WU ; Lei CHEN ; Zhihao YING ; Naiyong CHEN
Publication Type:Journal Article
Keywords: Epidermal growth factors; Adipose-derived stem cells; Apoptosis
From: Chinese Journal of Trauma 2010;26(5):468-471
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of epidermal growth factor (EGF) on the proliferation , migration and apoptosis of adipose-derived stem cells (ADSCs) in vitro. Methods Starved cell model and FasL-induced apoptosis model were established in serum-free media. The effects of 10 nmol/L and 100 nmol/L of EGF on the proliferation, migration and apoptosis of ADSCs in vitro were observed. The exrpessions of signal pathway proteins like phospholipaseC-γ(PLC-γ) , extracellular regulated kinase (ERK) and AKT were also detected. Results The proliferation, migration and anti-ap-optosis of ADSCs were promoted by 10 nmol/L or 100 nmol/L of EGF, and the expressions of PLC-'y, ERK and AKT were up-regulated. Conclusion EGF can promote the proliferation, migration and an-ti-apoptosis of adipose-derived ADSCs in vitro.